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Niimura, Nobuo; Arai, Shigeki; Kurihara, Kazuo; Chatake, Toshiyuki*; Tanaka, Ichiro*; Bau, R.*
Cellular and Molecular Life Sciences, 63(3), p.285 - 300, 2006/02
Times Cited Count:41 Percentile:37.86(Biochemistry & Molecular Biology)Neutron diffraction provides an experimental method of directly locating hydrogen atoms in proteins and DNA oligomers. Three different types of high resolution neutron diffractometers for biological macromolecules have been constructed in Japan, France and the U.S.A., and they have all been actively used in recent years to determine the crystal structures of numerous proteins. Examples include the detailed geometries of hydrogen bonds, information on H/D exchange in proteins, the unambiguous location of protons, the role of key hydrogen atoms in enzymatic activity and thermostability, and the dynamical behavior of hydration structures, all of which have been extracted from these structural results and reviewed in this article. Other important techniques, such as the optimization of growth of large single crystals using phase diagrams, the preparation of fully deuterated proteins, the introduction of cryogenic techniques to neutron protein crystallography, and the establishment of a "hydrogen and hydration in proteins" database, will also be described in this paper.
Nakamura, Kensuke; Go, Nobuhiro
Cellular and Molecular Life Sciences, 62(18), p.2050 - 2066, 2005/09
Times Cited Count:214 Percentile:86.8(Biochemistry & Molecular Biology)Multicopper blue proteins (MCBPs) are multi-domain proteins that utilize the distinctive redox ability of copper ions. There are variety of MCBPs that have been roughly classified into three different groups, (1)nitrite reductase-type with two domains, (2)laccase-type with three domains, and (3)ceruloplasmin-type with six domains. Together, the second and third group are often commonly called multicopper oxidases (MCOs). The rapid accumulation of genome sequence information in recent years has revealed several new types of proteins containing MCBP domains, mainly from bacteria. In this review, the recent research on the functions and structures of MCBPs is summarized, mainly focusing on the new types. The latter half of this review focusses on the two-domain MCBPs, which we propose as the evolutionary intermediate of the MCBP family.